[Latin Name]  Citrus aurantium L.

[Specification] Synephrine 4.0%–80%

[Appearance] Yellow brown powder

Plant Part Used: Fruit

[Particle size] 80Mesh

[Loss on drying] ≤5.0%

[Heavy Metal] ≤10PPM

[Storage] Store in cool & dry area, keep away from the direct light and heat.

[Shelf life] 24 Months

[Package] Packed in paper-drums and two plastic-bags inside.

[Net weight] 25kgs/drum

[What is Citrus Aurantium]

Citrus aurantium L, belonging to the family Rutaceae, is widely distributed in China. Zhishi, the Chinese traditional name for Citrus aurantium, has long been a folk medicine in traditional Chinese medicine (TCMto improve indigestion and help stimulate the Qi (energy force).

[Function]

1. Have the function of antioxidant, anti-inflammatory, hypolipidemic, vasoprotective and anticarcinogenic and cholesterol lowering actions.

2. Have the function of inhibiting following enzymes: Phospholipase A2, lipoxygenase, HMG-CoA reductase and cyclo-oxygenase.

3. Have the function of improving the health of capillaries by reducing the capillary permeability.

4. Have the function of reducing hay fever and other allergic conditions by inhibiting the release of histamine from mast cells. The possible activity of hesperidin could be explained by the inhibition of polyamine synthesis. (bitter orange extract)

This is Pandeeswari Jeeva’s application video for the Engage your Audience! 2017 contest, which she participated in during FEMS 2017.

Genetic engineering of Saccharomyces cerevisiae for hyaluronic acid production

Hyaluronic acid (HA) is vital for normal growth and development of vertebrate which makes it as a commercially valuable polysaccharide in pharmaceutical and cosmetics industries. HA is synthesized by polymerization of two nucleotide sugars UDP-glucuronic acid and UDP-N-acetylglucosamine by the enzyme HA synthase (hasA). Two major genes required for the formation of HA monomers are hasB (UDP-glucose dehydrogenase) and hasD (glucosamine-phosphate acetyl transferase) respectively. Native Streptococcal species are being used for industrial scale production of HA, however, the presence of endotoxins and pathogenic factors renders it unsuitable for medical purposes. Hence, this demands for metabolic engineering of Generally Recognized As Safe (GRAS) microbes to produce HA. We have chosen Saccharomyces cerevisiae as a host for HA production due to its advantages such as lack of pathogenecity and hyaluronidase. In addition, it does not produce HA or any other polysaccharide that contains glucuronic acid due to absence of hasA and hasB. Thus, it makes S. cerevisiae a superior model system to elucidate HA biosynthesis. Jeong et.al., (2014) has demonstrated the expression of has genes from Xenopus laevis, though with low yield, in Pichia pastoris for HA production. In the current work, we have expressed X. laevis has genes in S. cerevisiae for producing medical-grade HA.

Chocolate Pumpkin Muffins with Pumpkin Seeds Glaze are a great treat to have around for breakfast, lunch box treats, or after school snacks.
These muffins keep for 3 days at room temperature and up to a week in the fridge.

½ cup brown sugar (100g)
¾ cup milk (180g)
2 tbsp vegetable oil
2 cup flour (240g)
3½ tsp baking powder
5.6oz pumpkin (160g)
¼ cup pumpkin seeds

TOPPING:
pumpkin seeds
sunflower seeds

355°F – 180°C
15 minutes

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