[Latin Name] Griffonia simplicifolia

[Plant Source] Griffonia Seed

[Specifications] 98%; 99% HPLC

[Appearance] White fine powder

Plant Part Used: Seed

[Particle size] 80 Mesh

[Loss on drying] ≤5.0%

[Heavy Metal] ≤10PPM

[Pesticide residue] EC396-2005, USP 34, EP 8.0, FDA

[Storage] Store in cool & dry area, keep away from the direct light and heat.

[Shelf life] 24 Months

[Package] Packed in paper-drums and two plastic-bags inside.

[Net weight] 25kgs/drum

[What is 5-HTP]

5-HTP (5-Hydroxytryptophan) is a chemical by-product of the protein building block L-tryptophan. It is also produced commercially from the seeds of an African plant known as Griffonia simplicifolia 5-HTP is used for sleep disorders such as insomnia, depression, anxiety, migraine and tension-type headaches, fibromyalgia, obesity, premenstrual syndrome (PMS), premenstrual dysphoric disorder (PMDD), attention deficit-hyperactivity disorder (ADHD), seizure disorder, and Parkinson’s disease.

 [How does it work?]

5-HTP works in the brain and central nervous system by increasing the production of the chemical serotonin. Serotonin can affect sleep, appetite, temperature, sexual behavior, and pain sensation. Since 5-HTP increases the synthesis of serotonin, it is used for several diseases where serotonin is believed to play an important role including depression, insomnia, obesity, and many other conditions.

[Function]

Depression. Some clinical research shows that taking 5-HTP by mouth improve symptoms of depression in some people. Some clinical research shows that taking 5-HTP by mouth might be as beneficial as certain prescription antidepressant drugs for improving depression symptoms. In most studies, 150-800 mg daily of 5-HTP was taken. In some cases, higher doses have been used.

Down syndrome. Some research shows that giving 5-HTP to infants with Down syndrome might improve muscle and activity. Other research shows that it does not improve muscle or development when taken from infancy until 3-4 years of age. Research also shows that taking 5-HTP along with conventional prescription drugs does improve development, social skills, or language skills.

Anxiety 5-HTP was found to be protective against carbon dioxide-induced panic attacks. One study compared 5-HTP and the prescription medication clomipramine for anxiety. Clomipramine is a tricyclic antidepressant used for treating obsessive-compulsive disorder. 5-HTP was found to be somewhat effective in reducing anxiety symptoms, but not as effective as clomipramine.

Sleep 5-HTP supplements fared a little better for insomnia.5-HTP decreased the time required to get to sleep and decreased the number of nighttime awakenings. Taking 5-HTP along with GABA (gamma-aminobutyric acid), a relaxing neurotransmitter, reduced the time it took to fall asleep and increased the duration and quality of sleep. One study found that children with night terrors benefited from 5-HTP.

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Bacterial CultureMediabasics Dr.T.V.Rao MD
,Major Contribution to Culture Media ,Agar – Agar Frau Hesse’scontribution ,Agar – Agar Solid medium is made by adding Agar
Agar is obtained from Sea weeds New Zealand agar is more Agar contain long chain poly saccharides.Inoranic salts and protein like substance Melts at 980c and sets at 420c ,Agar – Agar Complex polysaccharide
Used as solidifying agent for culture media in Petri plates, slants, and deeps Generally not metabolized by microbes Liquefies at 98°C Solidifies ~42°C Dr.T.V.Rao MD’s ‘e’ learning series ,Media and Culture Media: Nutrients (agar, pH indicators, proteins and carbohydrates) used to grow organisms outside of their natural habitats
Culture: The propagation of microorganisms using various media ,Culture media Used to grow bacteria
Can be used to: Enrich the numbers of bacteria
Select for certain bacteria and suppress others Differentiate among different kinds of bacteria ,Culture and Medium Culture is the term given to microorganisms that are cultivated in the lab for the purpose of identifying and studying them.
Medium is the term given to the combination of ingredients that will support the growth and cultivation of microorganisms by providing all the essential nutrients required for the growth (that is, multiplication) in order to cultivate these microorganisms in large numbers to study them. ,Specific Media Defined media are media composed of pure ingredients in carefully measured concentrations dissolved in double distilled water i.e., the exact chemical composition of the medium is known. Typically, they contain a simple sugar as the carbon and energy source, an inorganic nitrogen source, various mineral salts and if necessary growth factors (purified amino acids, vitamins, purines and pyrimidines
,Need for Culture Media It is usually essential to obtain a culture by grwoing the organism in an artificial medium.
If more than one species or type of organism are present each requires to be carefully separated or isolated in pure culture. Several organism need the determination of Antibiotic sensitivity pattern for optimal antibiotic selection ,Basic requirements of culture media Nutrients – Energy source – Carbon source – Nitrogen source Mineral salts – Sulphate, phosphates, chlorides & carbonates of K, Mg & Ca. A suitable pH – 7.2 – 7.4 Accessory growth factors – Tryptophan for Salmonella typhi – X & V factors for H. influenzae
,Pouring the Culture Plates ,Petri dish with Media Plate: provide large surface for isolation and observation of colonies
Using a sterile loop or a sterile swab streak your sample on the petri plate Important let your sterilized loop cool before you pick up your sample ,Classification of Culture media Based on the consistency:Liquid — Peptone water, Nutrient broth Semisolid — Nutrient agar stabsSolid — Blood agar, Serum agar Based on Oxygen requirement: — Aerobic medium — Anaerobic media
,Aerobic Media Simple media Complex mediaMay be Synthetic or Defined Medium – Enriched media – Differential media – Enrichment media – Selective media Semisyntetic Medium – Sugar media – Transport media
,Aerobic media Liquid media – Peptone water(1% peptone +0.5%Nacl + 100 ml water)- Nutrient broth ( peptone water + 1% meat extract Solid media- Nutrient agar (nutrient broth + 2% Agar) Use: To grow non-fastidious microorganisms Simple media- consists of only basic necessities
,Liquid Medium Difficulat to identify all types of organisms
Suitable for isolation of bacteria from Blood culturing and water analysis ,Peptone Peptone contain partially digested proteins
Proteases Polypeptides Aminoacids Inorganic salts Phosphates Potassium and Magnesium Riboflavin Meat exract called as Lab lemco ,Nutrient Agar Contain 2% ag

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