Hot Shot Method of DNA Extraction – Amrita University


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DNA, the building block of life is the genetic material for all living organisms and it contains information that is crucial for heredity. DNA isolation is necessary for genetic analysis, which is used for scientific, medical, or forensic purposes. Presence of proteins, lipids, polysaccharides and some other organic or inorganic compounds in the DNA preparation can interfere with DNA analysis methods, especially with polymerase chain reaction (PCR).
DNA isolation from fish fins or scales provides a suitable non-invasive procedure which can overcome the difficulties encountered during tissue sampling from other tissues and allows the maintenance of the individuals without much discipline.


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