Mkpụrụ mkpụrụ greepu bụ ụdị polyphenols ewepụtara na mkpụrụ vine. Ihe mejupụtara ya bụ procyanidins, catechins, epicatechins, gallic acid, epicatechin gallate na polyphenols ndị ọzọ.
njirimara
Ike antioxidant
Mkpokọta mkpụrụ greepu bụ ihe okike dị ọcha. Ọ bụ otu n'ime antioxidants kachasị arụ ọrụ nke sitere na isi mmalite osisi. Nnwale ahụ gosipụtara na mmetụta antioxidant ya bụ 30 ~ 50 ugboro karịa vitamin C na vitamin E.
ọrụ
Procyanidins nwere ọrụ siri ike ma nwee ike igbochi carcinogens na sịga. Ikike ha iji weghara radicals free na mmiri mmiri bụ 2 ~ 7 ugboro dị elu karịa nke antioxidants izugbe, dị ka α- Ọrụ nke tocopherol dị elu karịa okpukpu abụọ.
wepụ
Achọpụtara na n'etiti ọtụtụ anụ ahụ osisi, ọdịnaya nke proanthocyanidins na mkpụrụ vaịn na mkpụrụ osisi pine bụ nke kachasị elu, na ụzọ ndị bụ isi nke iwepụta Proanthocyanidins sitere na mkpụrụ vaịn bụ mmịpụta ihe mgbaze, mmịpụta ngwa ndakwa nri, mmịpụta ultrasonic na mmịpụta CO2 dị oke egwu. Mkpụrụ grape proanthocyanidins wepụ nwere ọtụtụ adịghị ọcha, nke chọrọ nhụsianya ọzọ iji meziwanye ịdị ọcha nke proanthocyanidins. Ụzọ nchacha a na-ejikarị gụnyere mmịpụta ihe mgbaze, nzacha akpụkpọ ahụ na chromatography.
Ntinye uche nke ethanol nwere mmetụta kachasị na mwepu nke mkpụrụ vaịn proanthocyanidins, na oge mmịpụta na okpomọkụ enweghị mmetụta dị ịrịba ama na mwepu nke mkpụrụ vine proanthocyanidins. Oke mmịpụta kacha mma bụ ndị a: ntinye ethanol 70%, oge mmịpụta 120 min, oke mmiri siri ike 1:20.
Nnwale adsorption static na-egosi na ọnụego mgbasa ozi kachasị elu nke hpd-700 maka proanthocyanidins bụ 82.85%, sochiri da201, nke bụ 82.68%. Ọdịiche dị ntakịrị. Ọzọkwa, ikike adsorption nke resin abụọ a maka proanthocyanidins bụkwa otu ihe ahụ. N'ime ule desorption, da201 resin nwere ọnụego desorption kachasị elu nke procyanidins, nke bụ 60.58%, ebe hpd-700 nwere naanị 50.83%. Ejikọtara ya na nyocha adsorption na desorption, da210 resin kpebisiri ike ịbụ resin mgbasa ozi kacha mma maka nkewa nke procyanidins.
Site na njikarịcha usoro, mgbe ntinye nke proanthocyanidins bụ 0.15mg / ml, ọnụ ọgụgụ na-erugharị bụ 1ml / min, 70% ethanol ngwọta na-eji dị ka eluent, ọnụ ọgụgụ na-erute bụ 1ml / min, na ego nke eluent bụ 5bv, wepụ. nke mkpụrụ vine proanthocyanidins nwere ike ịsacha na mbụ.