Ukukhishwa kwembewu yamagilebhisi uhlobo lwama-polyphenols akhishwa embewini yamagilebhisi. Ikakhulukazi yakhiwa ama-procyanidin, ama-catechin, ama-epicatechin, i-gallic acid, i-epicatechin gallate namanye ama-polyphenols.
isici
Amandla e-Antioxidant
Imbewu yamagilebhisi ekhishwe iyinto yemvelo emsulwa. Ingenye yama-antioxidants asebenza kahle kakhulu avela emithonjeni yezitshalo. Ukuhlolwa kukhombisa ukuthi umphumela wawo wokulwa ne-antioxidant uphindwe izikhathi ezingama-30 ~ 50 kune-vitamin C no-vitamin E.
umsebenzi
I-Procyanidins inomsebenzi oqinile futhi ingavimbela i-carcinogens kugwayi. Ikhono labo lokubamba ama-radicals mahhala esigabeni esinamanzi yi-2 ~ 7 izikhathi ezingaphezu kwe-antioxidant evamile, njenge-α- Umsebenzi we-tocopherol ungaphezu kokuphindwe kabili.
khipha
Kutholakale ukuthi phakathi kwezicubu eziningi zezitshalo, okuqukethwe kwe-proanthocyanidins kumbewu yamagilebhisi kanye ne-pine bark extract kwakuphakeme kakhulu, futhi izindlela eziyinhloko zokukhipha i-Proanthocyanidin kusuka enzalweni yamagilebhisi kwakuyi-solvent extraction, i-microwave extraction, i-ultrasonic extraction kanye ne-supercritical CO2 extraction. Imbewu yamagilebhisi i-proanthocyanidin ekhishwe iqukethe ukungcola okuningi, okudinga ukuhlanzwa okwengeziwe ukuze kuthuthukiswe ubumsulwa be-proanthocyanidin. Izindlela zokuhlanza ezivame ukusetshenziswa zihlanganisa ukukhishwa kwe-solvent, ukuhlunga ulwelwesi kanye ne-chromatography.
Ukugcwala kwe-Ethanol kube nomthelela obaluleke kakhulu esilinganisweni sokukhishwa kwembewu yamagilebhisi i-proanthocyanidin, futhi isikhathi sokuyikhipha kanye nezinga lokushisa akubanga nomphumela obonakalayo esilinganisweni sokukhishwa kwembewu yamagilebhisi i-proanthocyanidin. Imingcele yokukhipha elungile yayimi kanje: ukuhlushwa kwe-ethanol 70%, isikhathi sokukhipha imizuzu engu-120, isilinganiso soketshezi oluqinile 1:20.
Ukuhlolwa kwe-adsorption emile kukhombisa ukuthi izinga eliphakeme kakhulu le-hpd-700 le-proanthocyanidins lingama-82.85%, lilandelwa yi-da201, elingu-82.68%. Mncane umehluko. Ngaphezu kwalokho, amandla e-adsorption alawa ma-resin amabili we-proanthocyanidins nawo ayafana. Ekuhlolweni kwe-desorption, i-da201 resin inezinga eliphakeme kakhulu lokuncibilika kwe-procyanidin, elingu-60.58%, kuyilapho i-hpd-700 ino-50.83% kuphela. Ihlanganiswe nokuhlolwa kwe-adsorption kanye ne-desorption, i-da210 resin inqunywe ukuthi iyona engcono kakhulu ye-adsorption resin yokuhlukaniswa kwama-procyanidin.
Ngokusebenzisa inqubo yokwenza kahle, lapho ukugcwala kwe-proanthocyanidin kungu-0.15mg/ml, izinga lokugeleza lingu-1ml/min, isisombululo se-ethanol esingu-70% sisetshenziswa njengenyezi, izinga lokugeleza lingu-1ml/min, kanti inani le-eluent lingu-5bv, ukukhishwa. Imbewu yamagilebhisi i-proanthocyanidin ingahlanzwa kuqala.