He ʻano polyphenols i unuhi ʻia mai nā hua waina. ʻO ka nui o nā procyanidins, catechins, epicatechins, gallic acid, epicatechin gallate a me nā polyphenols ʻē aʻe.
hiʻona
Hiki i ka antioxidant
He mea maʻemaʻe maoli ka hua waina. ʻO ia kekahi o nā antioxidants maikaʻi loa mai nā kumu mea kanu. Hōʻike ka hōʻike i kāna hopena antioxidant he 30 ~ 50 mau manawa o ka huaora C a me ka huaora E.
hana
He hana ikaika ko Procyanidins a hiki ke kaohi i ke kino make o ka paka. ʻO ko lākou hiki ke hopu i nā radical manuahi i ka pae wai he 2 ~ 7 mau manawa kiʻekiʻe ma mua o nā antioxidants maʻamau, e like me α- ʻO ka hana o ka tocopherol ma mua o ʻelua mau manawa.
unuhi
Ua ʻike ʻia ʻo ia ka mea kiʻekiʻe loa ma waena o nā mea kanu he nui, ʻo ka ʻike o ka proanthocyanidins i loko o ka hua waina a me ka ʻili paina ka mea kiʻekiʻe loa, a ʻo nā ʻano kumu nui o ka unuhi ʻana i nā Proanthocyanidins mai nā hua waina, ʻo ia ka hoʻoheheʻe ʻana, microwave extraction, ultrasonic extraction a supercritical CO2 extraction. ʻO ka hua waina proanthocyanidins extract he nui nā mea haumia, pono e hoʻomaʻemaʻe hou aku e hoʻomaikaʻi i ka maʻemaʻe o nā proanthocyanidins. ʻO nā ʻano hoʻomaʻemaʻe maʻamau i hoʻohana ʻia me ka wehe ʻana i ka solvent, ka kānana membrane a me ka chromatography.
ʻO ka manaʻo ethanol ka hopena nui loa i ka unuhi ʻana o ka hua waina proanthocyanidins, a ʻaʻohe hopena koʻikoʻi o ka manawa a me ka mahana i ka unuhi ʻana o nā hua waina proanthocyanidins. ʻO nā ʻāpana hoʻokaʻawale maikaʻi loa e like me kēia: ka hoʻonui ʻana i ka ethanol 70%, ka manawa unuhi 120 min, ka ratio paʻa-wai 1:20.
Hōʻike ka hoʻokolohua static adsorption i ka helu adsorption kiʻekiʻe loa o hpd-700 no nā proanthocyanidins he 82.85%, a ukali ʻia e da201, ʻo ia ka 82.68%. He ʻokoʻa iki. Eia kekahi, ʻo ka mana adsorption o kēia mau resins ʻelua no ka proanthocyanidins like pū kekahi. I ka ho'āʻo desorption, loaʻa i ka da201 resin ka helu desorption kiʻekiʻe loa o nā procyanidins, ʻo ia ka 60.58%, aʻo hpd-700 wale nō ka 50.83%. Hoʻohui ʻia me nā hoʻokolohua adsorption a me desorption, ua hoʻoholo ʻia ka resin da210 e lilo i resin adsorption maikaʻi loa no ka hoʻokaʻawale ʻana o nā procyanidins.
Ma o ke kaʻina hana optimization, inā he 0.15mg/ml ka nui o ka proanthocyanidins, he 1ml / min ke kahe kahe, hoʻohana ʻia ʻo 70% ethanol solution ma ke ʻano he eluent, he 1ml / min ka kahe kahe, a ʻo ka nui o ka eluent he 5bv, hiki ke hoʻomaʻemaʻe mua ʻia ka extract o ka hua waina proanthocyanidins.